Rabbit Polyclonal Antibody to IkB-a(Phospho-Ser32/Ser36)
货号：
P40147
别名：
I-kappa-B-alpha; IKBA; NF-kappaB inhibitor alpha; NFKBI; NFKBIA
应用：
WB,IHC,IF
反应种属：
Human,Mouse
抗体类型：
Primary antibody
Swissprot：
P25963
规格：
目录价
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Description |
|---|
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription. |
References |
|---|
| Shieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823.Honda, R. et al. (1997) FEBS Lett. 420, 25-27.Hirao, A. et al. (2000) Science 287, 1824-1827.Tibbetts, R. S. et al. (1999) Genes Dev. 13, 152-157.Chehab,N.H.et al. (1999) Proc .Natl .Acad. Sci. USA 96, 13777-13782. |
Specification |
|
|---|---|
| Aliases | I-kappa-B-alpha; IKBA; NF-kappaB inhibitor alpha; NFKBI; NFKBIA |
| Entrez GeneID | 4792; |
| Swissprot | P25963 |
| WB Predicted band size | 39kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human IkB-a. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| IF | 1/100-1/200 |
Product Image
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Western blot analysis of extracts from Hela cells untreated or treated with TNF using IkB-a(Phospho-Ser32/Ser36) Antibody P40147.
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Immunofluorescence staining of methanol-fixed Hela cells using IkB-a(Phospho-Ser32/Ser36) Antibody P40147.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-a(Phospho-Ser32/Ser36) Antibody P40147(left) or the same antibody preincubated with blocking peptide(right).
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