Rabbit Polyclonal Antibody to MEK1(Phospho-Ser221)
货号：
P40155
别名：
ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1
应用：
WB,IHC,IF
反应种属：
Human,Mouse,Rat
抗体类型：
Primary antibody
Swissprot：
Q02750
规格：
目录价
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Description |
|---|
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. |
References |
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| Zebisch A, et al. (2006) Cancer Res; 66(7): 3401-8. Luciano BS, et al. (2006)J Biol Chem; 279(50): 52117-23. Wang X, et al. (2003) Oncogene; 22(1): 109-16. Gopalbhai K, et al. (2003) J Biol Chem; 278(10): 8118-25. Ling MT, et al. (2002)Oncogene; 21(55): 8498-505. |
Specification |
|
|---|---|
| Aliases | ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1 |
| Entrez GeneID | 5604; |
| Swissprot | Q02750 |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 221 (A-N-S(p)-F-V) derived from Human MEK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| IF | 1/100-1/200 |
Product Image
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Western blot analysis of extracts from Hela cells untreated or treated with EGF using MEK1(Phospho-Ser221) Antibody P40155.
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Western blot analysis of extracts from Hela cells, treated with calf intestinal phosphatase (CIP), using MEK1 (Phospho-Ser221) Antibody P40155.
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Immunofluorescence staining of methanol-fixed Hela cells using MEK1(Phospho-Ser221) Antibody P40155.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1(Phospho-Ser221) Antibody P40155(left) or the same antibody preincubated with blocking peptide(right).
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