Rabbit Polyclonal Antibody to eIF4E(Phospho-Ser209)
货号：
P40219
别名：
mRNA cap-binding protein; eIF-4F 25 kDa subunit;
应用：
WB,IHC,IF
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P06730
规格：
目录价
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Description |
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Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. |
References |
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| Li BD, et al. (1998) Ann Surg; 227(5): 756-763 Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520 De Gregorio E, et al. (2001) RNA; 7(1): 106-113 Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461 |
Specification |
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|---|---|
| Aliases | mRNA cap-binding protein; eIF-4F 25 kDa subunit; |
| Entrez GeneID | 1977; |
| Swissprot | P06730 |
| WB Predicted band size | 25kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| IF | 1/100-1/200 |
Product Image
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Western blot analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using eIF4E (Phospho-Ser209) Antibody P40219.
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Immunofluorescence staining of methanol-fixed MCF cells using eIF4E(Phospho-Ser209) Antibody P40219.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E(Phospho-Ser209) Antibody P40219(left) or the same antibody preincubated with blocking peptide(right).
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