Rabbit Polyclonal Antibody to ACK1 (Phospho-Tyr284)
货号：
P40385
别名：
ACK1; Activated p21cdc42Hs kinase; EC 2.7.10.2; kinase ACK1;
应用：
WB,IHC,IF
反应种属：
Human,Mouse
抗体类型：
Primary antibody
Swissprot：
Q07912
规格：
目录价
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Description |
|---|
Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Manser E., Nature 363:364-367(1993)Ota T., Nat. Genet. 36:40-45(2004)Eisenmann K.M., Nat. Cell Biol. 1:507-513(1999) |
Specification |
|
|---|---|
| Aliases | ACK1; Activated p21cdc42Hs kinase; EC 2.7.10.2; kinase ACK1; |
| Entrez GeneID | 10188; |
| Swissprot | Q07912 |
| WB Predicted band size | 120kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 284 (D-H-Y(p)-V-M) derived from Human ACK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| IF | 1/100-1/200 |
Product Image
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Western blot analysis of extracts from HepG2 cells treated with EGF using ACK1 (Phospho-Tyr284) Antibody P40385.The lane on the right is treated with the antigen-specific peptide.
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Immunofluorescence staining of methanol-fixed A549 cells using ACK1 (Phospho-Tyr284) Antibody P40385.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody P40385 (left)or the same antibody preincubated with blocking peptide (right).
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