Rabbit Polyclonal Antibody to WEE1 (Phospho-Ser642)
货号：
P40414
别名：
WEE1A; WEE1hu; kinase WEE1;
应用：
WB
反应种属：
Human,Mouse,Rat
抗体类型：
Primary antibody
Swissprot：
P30291
规格：
目录价
在线咨询
Description |
|---|
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation |
References |
|---|
| Watanabe N., EMBO J. 14:1878-1891(1995) Cichutek A., Cytogenet. Cell Genet. 93:277-283(2001) Igarashi M., Nature 353:80-83(1991) |
Specification |
|
|---|---|
| Aliases | WEE1A; WEE1hu; kinase WEE1; |
| Entrez GeneID | 7465; |
| Swissprot | P30291 |
| WB Predicted band size | 100kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
鄂公网安备42018502007531号
