Mouse Monoclonal Antibody to CASP6
货号:
31730
别名:
MCH2
应用:
IF,FCM
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
P55212
规格:
目录价
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Description |
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This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. |
References |
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1.Biochemistry. 2017 Aug 29;56(34):4568-4577. 2.Acta Neuropathol Commun. 2016 Dec 8;4(1):127. |
Specification |
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Aliases | MCH2 |
Entrez GeneID | 839 |
Swissprot | P55212 |
clone | 3B7E10 |
WB Predicted band size | 33.3kDa |
Host/Isotype | Mouse IgG2a |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human CASP6 (AA: 194–293) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
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IF | 1/100 - 1/500 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Immunofluorescence analysis of Hela cells using CASP6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of Hela cells using CASP6 mouse mAb (green) and negative control (red).