Mouse Monoclonal Antibody to 4E-BP1/2/3 (Phospho-Thr 45)
货号：
P41229
别名：
4E-BP1 antibody</br> 4EBP1 antibody</br> 4EBP1_HUMAN antibody</br> BP 1 antibody</br> eIF4E binding protein 1 antibody</br> eIF4E-binding protein 1 antibody</br> Eif4ebp1 antibody</br> Eukaryotic translation initiation factor 4E-binding protein 1 antibody
应用：
WB,IP
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
Q13541
规格：
目录价
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Description |
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The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5' end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). 4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyper-phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3-kinase/Akt pathway and the FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues and phosphorylation by FRAP/mTOR on Threonine 37 and Threonine 46 of human 4E-BP1 may prime it for sub-sequent phosphorylation at sites including Serine 65 and Threonine 70. The corresponding rat residues include Threonine 36, Threonine 45, Serine 64 and Threonine 69. In vitro, 4E-BP1 is also phosphorylated by ataxia telangiectasia (ATM) at human Serine 112 (rat Serine 111) in response to an increase in insulin levels. |
Specification |
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|---|---|
| Aliases | 4E-BP1 antibody 4EBP1 antibody 4EBP1_HUMAN antibody BP 1 antibody eIF4E binding protein 1 antibody eIF4E-binding protein 1 antibody Eif4ebp1 antibody Eukaryotic translation initiation factor 4E-binding protein 1 antibody PHAS-I antibody PHASI antibody Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody |
| Entrez GeneID | 1978 |
| Swissprot | Q13541 |
| WB Predicted band size | 21kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | peptide |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
Application |
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|---|---|
| WB | 1/1000-1/1000 |
| IP | 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) |
Product Image
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Westernblot analysis of 4E-BP1/2/3 phosphorylation inuntreated (A), calyculinA treated (B) and calyculin A and lambda protein phosphatase treated (C) Jurkat whole cell lysates.
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Western blot analysis of 4E-BP1 phosphorylation in nontransfected (A,D), untreated human 4E-BP1 transfected (B,E) and lambda protein phosphatase treated human 4E-BP1 transfected (C,F) 293T whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (A,B,C) and 4E-BP1 (D,E,F).
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