Rabbit Polyclonal Antibody to MKI67IP (phospho-Thr234)
货号：
P41227
别名：
MKI67IP; NOPP34; MKI67 FHA domain-interacting nucleolar phosphoprotein; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI-67; hNIFK
应用：
WB,IHC,IF
反应种属：
Human,Mouse,Rat
抗体类型：
Primary antibody
Swissprot：
Q9BYG3
规格：
目录价
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Specification |
|
|---|---|
| Aliases | MKI67IP; NOPP34; MKI67 FHA domain-interacting nucleolar phosphoprotein; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI-67; hNIFK |
| Entrez GeneID | 84365; |
| Swissprot | Q9BYG3 |
| WB Predicted band size | 34kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MKI67IP. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| IF | 1/100-1/300 |
Product Image
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Western blot analysis of MKI67IP (phospho-Thr234) expression in HeLa, NIH3T3, PC12 whole cell lysates.
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Immunofluorescent analysis of MKI67IP (phospho-Thr234) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Immunohistochemical analysis of MKI67IP (phospho-Thr234) staining in human brain formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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