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Rabbit Polyclonal Antibody to NFkB-p65(Ab-276)
货号: P41247
别名: NFKB3; RELA; TF65; Transcription factor p65; p65
应用: WB,IHC,IF
反应种属: Human,Mouse,Rat
抗体类型: Primary antibody
Swissprot: Q04206
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Description

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex

References

Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179.Baeuerle P A, et al. (1996) Cell 87:13-20.Haskill S, et al. (1991) Cell 65:1281-1289.Thompson J E, et al. (1995) Cell 80: 573-582.

Specification

Aliases NFKB3; RELA; TF65; Transcription factor p65; p65
Entrez GeneID 5970;
Swissprot Q04206
WB Predicted band size 65kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen Peptide sequence around aa.274~278 (R-P-S-D-R) derived from Human NFkB-p65.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500-1/1000
IHC 1/50-1/100
IF 1/100-1/200
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