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Rabbit Polyclonal Antibody to Chk1(Ab-317)
货号: P41334
别名: CHEK1
应用: WB,IHC
反应种属: Human
抗体类型: Primary antibody
Swissprot: O14757
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Description

Chek1 is a protein kinase that inhibits mitotic entry after DNA damage, required for the DNA damage checkpoint and is strongly similar to murine Chek1. Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The mouse and human proteins share 90% sequence identity through the protein kinase domains. The sequence of the 476-amino acid human Chek1 protein is 29%, 40%, and 44% identical to those of the fission yeast Chek1, C. elegans Chek1, and Drosophila 'grapes' (Grp) proteins, respectively. Chek1 is expressed ubiquitously as an approximately 2.4-kb mRNA, with the most abundant expression in thymus, testis, small intestine, and colon. The protein has altered mobility when isolated from cells treated with ionizing radiation, indicating that Chek1 is modified in response to DNA damage. In vitro, Chek1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, Chek1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-Cyclin-B complex and mitotic entry

References

Zhang YW, et al. (2005) Mol Cell; 19(5): 607-18. Bhoumik A, et al. (2005) Mol Cell; 18(5): 577-87. Rocha S, et al. (2005) EMBO J. Clarke CA, et al. (2005) Biochem J. Yu X, et al. (2004) Mol Cell Biol; 24(21): 9478-86.

Specification

Aliases CHEK1
Entrez GeneID 1111;
Swissprot O14757
WB Predicted band size 56kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Peptide sequence around aa.315~319 (S-S-S-Q-P) derived from Human Chk1.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500-1/1000
IHC 1/50-1/100
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