Rabbit Polyclonal Antibody to AMPKα1/AMPKα2 (Ab-183/172)
货号：
P41397
别名：
AMPK, AMPKa1
应用：
WB,IHC,IF
反应种属：
Human,Mouse,Rat
抗体类型：
Primary antibody
Swissprot：
Q13131
规格：
目录价
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Description |
|---|
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. |
References |
|---|
| Hurley RL, et al. (2005) J Biol Chem. Aug 12; 280(32): 29060-29066 Woods A, et al. (2003) Curr Biol. Nov 11; 13(22): 2004-2008 Nielsen JN, et al. (2003) J Appl Physiol. Feb; 94(2): 631-641 Da Silva Xavier G, et al. (2000) Proc Natl Acad Sci U S A. Apr 11; 97(8): 4023-4028. |
Specification |
|
|---|---|
| Aliases | AMPK, AMPKa1 |
| Entrez GeneID | 5562; |
| Swissprot | Q13131 |
| WB Predicted band size | 63kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa. 181~185/170~174 (L-R-T-S-C) derived from Human AMPKα1/AMPKα2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| IF | 1/100-1/200 |
Product Image
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Western blot analysis of extracts from A549, HepG2 and 3T3 cells using AMPKα1/AMPKα2 (Ab-183/172) Antibody P41397.
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Immunofluorescence staining of methanol-fixed Hela cells using AMPKα1/AMPKα2 (Ab-183/172) Antibody P41397.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AMPKα1/AMPKα2 (Ab-183/172) Antibody P41397(left) or the same antibody preincubated with blocking peptide(right).
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