Rabbit Polyclonal Antibody to AMPKα1/AMPKα2 (Ab-183/172)
货号:
P41397
别名:
AMPK, AMPKa1
应用:
WB,IHC,IF
反应种属:
Human,Mouse,Rat
抗体类型:
Primary antibody
Swissprot:
Q13131
规格:
目录价
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Description |
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Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. |
References |
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Hurley RL, et al. (2005) J Biol Chem. Aug 12; 280(32): 29060-29066 Woods A, et al. (2003) Curr Biol. Nov 11; 13(22): 2004-2008 Nielsen JN, et al. (2003) J Appl Physiol. Feb; 94(2): 631-641 Da Silva Xavier G, et al. (2000) Proc Natl Acad Sci U S A. Apr 11; 97(8): 4023-4028. |
Specification |
|
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Aliases | AMPK, AMPKa1 |
Entrez GeneID | 5562; |
Swissprot | Q13131 |
WB Predicted band size | 63kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | Peptide sequence around aa. 181~185/170~174 (L-R-T-S-C) derived from Human AMPKα1/AMPKα2. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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WB | 1/500-1/1000 |
IHC | 1/50-1/100 |
IF | 1/100-1/200 |
Product Image
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Western blot analysis of extracts from A549, HepG2 and 3T3 cells using AMPKα1/AMPKα2 (Ab-183/172) Antibody P41397.
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Immunofluorescence staining of methanol-fixed Hela cells using AMPKα1/AMPKα2 (Ab-183/172) Antibody P41397.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AMPKα1/AMPKα2 (Ab-183/172) Antibody P41397(left) or the same antibody preincubated with blocking peptide(right).