Rabbit Polyclonal Antibody to Histone H2A.X(Ab-139)
货号：
P41455
别名：
H2A.X; H2AFX; H2a/x; HIST5-2AX;
应用：
WB,IHC,IF
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P16104
规格：
目录价
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Description |
|---|
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation |
References |
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| Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330.Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6 |
Specification |
|
|---|---|
| Aliases | H2A.X; H2AFX; H2a/x; HIST5-2AX; |
| Entrez GeneID | 3014; |
| Swissprot | P16104 |
| WB Predicted band size | 15kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.137~141 (Q-A-S-Q-E) derived from Human Histone H2A.X. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| IF | 1/100-1/200 |
Product Image
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Western blot analysis of extracts from HepG2 and 293 cells using Histone H2A.X(Ab-139) Antibody P41455.
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Immunofluorescence staining of methanol-fixed Hela cells using Histone H2A.X(Ab-139) Antibody P41455.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H2A.X(Ab-139) Antibody P41455(left) or the same antibody preincubated with blocking peptide(right).
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