Rabbit Polyclonal Antibody to MDM2(Ab-166)
货号:
P41568
别名:
HDMX, hdm2
应用:
WB,IHC,IF
反应种属:
Human,Mouse,Rat Monkey
抗体类型:
Primary antibody
Swissprot:
Q00987
规格:
目录价
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Description |
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This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues |
References |
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Haupt, Y. et al. (1997) Nature 387, 296-299. Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981. Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415. Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603. |
Specification |
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Aliases | HDMX, hdm2 |
Entrez GeneID | 4193; |
Swissprot | Q00987 |
WB Predicted band size | 90kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat Monkey |
Immunogen | Peptide sequence around aa.164~168 (A-T-S-E-T) derived from Human MDM2. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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WB | 1/500-1/1000 |
IHC | 1/50-1/100 |
IF | 1/100-1/200 |
Product Image
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Western blot analysis of extracts from HT29, Hela and 293 cells using MDM2(Ab-166) Antibody P41568.
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Immunofluorescence staining of methanol-fixed Hela cells using MDM2(Ab-166) Antibody P41568.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2(Ab-166) Antibody P41568(left) or the same antibody preincubated with blocking peptide(right).