Rabbit Polyclonal Antibody to p14 ARF
货号:
P41826
别名:
CDK4I; CMM2; MLM; MTS1; TP16
应用:
WB,IHC,IF
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
P42771
规格:
目录价
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Description |
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Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform 6 may be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform isstabilized by C1QBP. UniProtKB Q64364B篓娄atrice Eymin, Mol. Cell. Biol., Jun 2006; 26: 4339 - 4350. Guillaume Normand, J. Biol. Chem., Feb 2005; 280: 7118 - 7130. Abel S篓垄nchez-Aguilera, Blood, Feb 2002; 99: 1411 - 1418. Manel Esteller, Cancer Res., Apr 2001; 61: 2816 - 2821. |
Specification |
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Aliases | CDK4I; CMM2; MLM; MTS1; TP16 |
Entrez GeneID | 1029; |
Swissprot | P42771 |
WB Predicted band size | 18kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Synthesized peptide derived from human p14 ARF antibody. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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WB | 1/500-1/3000 |
IHC | 1/50-1/100 |
IF | 1/100-1/500 |
Product Image
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Western blot analysis of extracts from HeLa cells, using p14 ARF antibody P41826.
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Western blot analysis of extracts from 293 cells (Lane 2), using p14 ARF antiobdy P41826. The lane on the left is treated with synthesized peptide.
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Immunofluorescence analysis of HeLa cells, using p14 ARF antibody P41826.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using p14 ARF antibody P41826.