Rabbit Polyclonal Antibody to p22
货号：
P43621
别名：
C20orf74; KIAA1272; Ral GTPase-activating protein subunit alpha-2; 250 kDa substrate of Akt; AS250; p220
应用：
WB,IHC,IF
反应种属：
Human,Mouse,Rat
抗体类型：
Primary antibody
Swissprot：
Q2PPJ7
规格：
目录价
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Specification |
|
|---|---|
| Aliases | C20orf74; KIAA1272; Ral GTPase-activating protein subunit alpha-2; 250 kDa substrate of Akt; AS250; p220 |
| Entrez GeneID | 57186; |
| Swissprot | Q2PPJ7 |
| WB Predicted band size | 250kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RALGAPA2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| IF | 1/100-1/300 |
Product Image
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Western blot analysis of POLR3A expression in K562, MCF7, COS7 whole cell lysates.
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Immunofluorescent analysis of p220 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Immunohistochemical analysis of p220 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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