Rabbit Polyclonal Antibody to NT5C3
货号:
P43614
别名:
NT5C3; P5N1; UMPH1; Cytosolic 5'-nucleotidase 3A; Cytosolic 5'-nucleotidase 3; Cytosolic 5'-nucleotidase III; cN-III; Pyrimidine 5'-nucleotidase 1; P5'N-1; P5N-1; PN-I; Uridine 5'-monophosphate hydrolase 1; p36
应用:
WB,IHC,IF
反应种属:
Human,Mouse
抗体类型:
Primary antibody
Swissprot:
Q9H0P0
规格:
目录价
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Specification |
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Aliases | NT5C3; P5N1; UMPH1; Cytosolic 5'-nucleotidase 3A; Cytosolic 5'-nucleotidase 3; Cytosolic 5'-nucleotidase III; cN-III; Pyrimidine 5'-nucleotidase 1; P5'N-1; P5N-1; PN-I; Uridine 5'-monophosphate hydrolase 1; p36 |
Entrez GeneID | 51251; |
Swissprot | Q9H0P0 |
WB Predicted band size | 38kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NT5C3. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
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WB | 1/500-1/1000 |
IHC | 1/50-1/100 |
IF | 1/100-1/300 |
Product Image
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Western blot analysis of MRPS7 expression in rat tongue whole cell lysates.
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Immunofluorescent analysis of NT5C3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Immunohistochemical analysis of NT5C3 staining in human brain formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.