Rabbit Polyclonal Antibody to RAD51C
货号：
P43658
别名：
RAD51L2; DNA repair protein RAD51 homolog 3; R51H3; RAD51 homolog C; RAD51-like protein 2
应用：
WB,IHC,IF
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
O43502
规格：
目录价
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Specification |
|
|---|---|
| Aliases | RAD51L2; DNA repair protein RAD51 homolog 3; R51H3; RAD51 homolog C; RAD51-like protein 2 |
| Entrez GeneID | 5889; |
| Swissprot | O43502 |
| WB Predicted band size | 42kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RAD51C. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| IF | 1/100-1/300 |
Product Image
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Western blot analysis of OLFML2A expression in HepG2, A549 whole cell lysates.
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Immunofluorescent analysis of RAD51C staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Immunohistochemical analysis of RAD51C staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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