Rabbit Polyclonal Antibody to PAR4
货号:
P43672
别名:
PAR4; Proteinase-activated receptor 4; PAR-4; Coagulation factor II receptor-like 3; Thrombin receptor-like 3
应用:
WB,IF
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
Q96RI0
规格:
目录价
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Specification |
|
---|---|
Aliases | PAR4; Proteinase-activated receptor 4; PAR-4; Coagulation factor II receptor-like 3; Thrombin receptor-like 3 |
Entrez GeneID | 9002; |
Swissprot | Q96RI0 |
WB Predicted band size | 41kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PAR4. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
---|---|
WB | 1/500-1/1000 |
IF | 1/100-1/300 |
Product Image
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Western blot analysis of MRPL51 expression in HEK293T, NIH3T3 whole cell lysates.
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Immunofluorescent analysis of PAR4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).