Rabbit Polyclonal Antibody to VPS72
货号：
P43682
别名：
TCFL1; YL1; Vacuolar protein sorting-associated protein 72 homolog; Protein YL-1; Transcription factor-like 1
应用：
WB,IHC,IF
反应种属：
Human,Mouse
抗体类型：
Primary antibody
Swissprot：
Q15906
规格：
目录价
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Specification |
|
|---|---|
| Aliases | TCFL1; YL1; Vacuolar protein sorting-associated protein 72 homolog; Protein YL-1; Transcription factor-like 1 |
| Entrez GeneID | 6944; |
| Swissprot | Q15906 |
| WB Predicted band size | 41kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human VPS72. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| IF | 1/100-1/300 |
Product Image
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Western blot analysis of Cadherin 24 expression in SHSY5Y whole cell lysates.
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Immunofluorescent analysis of VPS72 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Immunohistochemical analysis of VPS72 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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