Mouse Monoclonal Antibody to NDC80
货号：
32242
别名：
HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
应用：
WB,IHC,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
O14777
规格：
目录价
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Description |
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This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011] |
References |
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| 1.J Biol Chem.2019 Jan 11;294(2):576-592. 2.Mol Biol Cell.2020 Jul 1;31(14):1453-1473. |
Specification |
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|---|---|
| Aliases | HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80 |
| Entrez GeneID | 10403 |
| Swissprot | O14777 |
| clone | 4B12H3 |
| WB Predicted band size | 74kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NDC80 (AA: 443-642) expressed in Mammalian. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Western blot analysis using NDC80 mouse mAb against Jurkat (1) cell lysate.
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Flow cytometric analysis of Hela cells using NDC80 mouse mAb (green) and negative control (red).
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Flow cytometric analysis of HepG2 cells using NDC80 mouse mAb (green) and negative control (red).
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Flow cytometric analysis of Jurkat cells using NDC80 mouse mAb (green) and negative control (red).
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Flow cytometric analysis of K562 cells using NDC80 mouse mAb (green) and negative control (red).
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using NDC80 mouse mAb with DAB staining.
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Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using NDC80 mouse mAb with DAB staining.
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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using NDC80 mouse mAb with DAB staining.
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Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using NDC80 mouse mAb with DAB staining.
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