Mouse Monoclonal Antibody to NEDD9
货号:
32248
别名:
CAS2; CASL; HEF1; CAS-L; CASS2
应用:
WB,IF,FCM
反应种属:
Human, Rat
抗体类型:
Primary antibody
Swissprot:
Q14511
规格:
目录价
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Description |
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The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
References |
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| 1.Biomolecules.2018 Dec 10;8(4):169. 2.Biochem Biophys Res Commun.2019 Jan 29;509(1):201-208. |
Specification |
|
|---|---|
| Aliases | CAS2; CASL; HEF1; CAS-L; CASS2 |
| Entrez GeneID | 4739 |
| Swissprot | Q14511 |
| clone | 3F7A9 |
| WB Predicted band size | 92.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Purified recombinant fragment of human NEDD9 (AA: 82-398) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

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Western blot analysis using NEDD9 mouse mAb against MCF-7 (1), Hela (2), C2C12 (3),and Hek293 (4) cell lysate.

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Immunofluorescence analysis of Hela cells using NEDD9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

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Flow cytometric analysis of HepG2 cells using NEDD9 mouse mAb (green) and negative control (red).

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Flow cytometric analysis of Jurkat cells using NEDD9 mouse mAb (green) and negative control (red).

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Flow cytometric analysis of K562 cells using NEDD9 mouse mAb (green) and negative control (red).







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