Mouse Monoclonal Antibody to CD30
货号：
32290
别名：
Ki-1; D1S166E,TNFRSF8
应用：
WB,IF,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
P28908
规格：
目录价
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Description |
|---|
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
References |
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| 1.J Clin Exp Hematop. 2020 Mar 28;60(1):19-23. 2.Biomarkers. 2020 Feb;25(1):69-75. |
Specification |
|
|---|---|
| Aliases | Ki-1; D1S166E,TNFRSF8 |
| Entrez GeneID | 943 |
| Swissprot | P28908 |
| clone | 1B1A4G2 |
| WB Predicted band size | 63.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Purified recombinant fragment of human CD30 (AA: 416-595) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IF | 1/50 - 1/200 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Western blot analysis using CD30 mouse mAb against Hela (1), MCF-7 (2), HCT116 (3),and PC-3 (4)cell lysate.
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Immunofluorescence analysis of Hela cells using CD30 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of Jurkat cells using CD30 mouse mAb (green) and negative control (red).
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