Rabbit Polyclonal Antibody to DFFA
货号:
P00440
别名:
DFF1; ICAD; DFF-45
应用:
WB,IHC
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
O00273
规格:
目录价
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Description |
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Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
Specification |
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Aliases | DFF1; ICAD; DFF-45 |
Swissprot | O00273 |
WB Predicted band size | 37 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Fusion protein of human DFFA |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
Application |
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WB | 1/500-1/2000 |
IHC | 1/30-1/150 |
ELISA | 1/5000-1/10000 |
Product Image
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Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Jurkat cell lysate, Primary antibody: P00440(DFFA Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
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The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P00440(DFFA Antibody) at dilution 1/40. (Original magnification: ×200)
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The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P00440(DFFA Antibody) at dilution 1/40. (Original magnification: ×200)