Mouse Monoclonal Antibody to beta-Actin
货号：
20270
别名：
PS1TP5BP1; ACTB
应用：
WB,IP,IF,FCM
反应种属：
Human,Mouse,Hamster,Rat,Monkey,Dog
抗体类型：
Loading Control Antibody
Swissprot：
P60709
规格：
目录价
在线咨询
Description |
|---|
Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. |
References |
|---|
| 1. Proteomics. 2005 Oct;5(15):3876-84. 2. PLoS Med. 2005 Oct;2(10):e263. 3. Mol Biol Cell. 2005 Nov;16(11):5055-60 4. Nature. 2005 Oct 20;437(7062):1173-8. |
Specification |
|
|---|---|
| Aliases | PS1TP5BP1; ACTB |
| Entrez GeneID | 60 |
| Swissprot | P60709 |
| clone | 8H10D10 |
| WB Predicted band size | 42kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Loading Control Antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Hamster,Rat,Monkey,Dog |
| Immunogen | Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/10000 - 1/40000 |
| IP | 1/100-1/200 |
| IF | 1/50 - 1/500 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
-
Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
-
Western blot analysis using beta-Actin mouse mAb against Rat serum(1),Rabbit serum(2),PC-12(3),Raw264.7(4),NIH/3T3(5),NRK(6),C2C12(7),C6(8),L1210(9),F9(10),CHO3D10(11),Hela(12),Jurkat(13) cell lysate.
-
Immunoprecipitation using beta-Actin mouse mAb(dilution: 1/250) against HL-60,C2C12,NRK,RSC,Hela,Cos7 cell lysate.
-
Confocal Immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin mouse mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
-
Flow cytometric analysis of MCF-7 cells using beta Actin mouse mAb (right) and negative control (left).
鄂公网安备42018502007531号
