Mouse Monoclonal Antibody to Cytokeratin 8
货号：
20280
别名：
CK8; CYK8; K2C8; KRT8
应用：
WB,IHC,IF
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P05787
规格：
目录价
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Description |
|---|
Cytokeratin 8, also known as CK8, KRT8, K8. Entrez Protein NP_002264. It is a member of the type II keratin family.Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells.It typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. |
References |
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| 1. Cancer Genet Cytogenet. 2007 Oct 15;178(2):94-103. 2. Cancer Lett. 2008 Jul 8;265(2):188-96. |
Specification |
|
|---|---|
| Aliases | CK8; CYK8; K2C8; KRT8 |
| Entrez GeneID | 3856 |
| Swissprot | P05787 |
| clone | 8A5D12 |
| WB Predicted band size | 54kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| IF | 1/200 - 1/1000 |
| ELISA | 1/10000 |
Product Image
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Western blot analysis using CK8 mouse mAb against A549 (1), Hela (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate.
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Confocal Immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using CK8 mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), lung cancer (B) and ovarian cancer tissue (C), showing membrane and cytoplasmic localization with DAB staining using CK8 mouse mAb.
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Immunohistochemical analysis of paraffin-embedded human Kidney tissues using CK8 mouse mAb
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