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Mouse Monoclonal Antibody to NANOG
货号: 20289
别名: NANOG
应用: WB,IF
反应种属: Human
抗体类型: Primary antibody
Swissprot: Q9H9S0
规格:
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Description

NANOG: Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.

References

1. Genes Cells. 2006 Sep;11(9):1115-23.
2. Mol Biol Cell. 2007 May;18(5):1543-53.

Specification

Aliases NANOG
Entrez GeneID 79923
Swissprot Q9H9S0
clone 1E6C4
WB Predicted band size 35kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500 - 1/2000
IF 1/200 - 1/1000
ELISA 1/10000
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