Mouse Monoclonal Antibody to NANOG
货号:
20289
别名:
NANOG
应用:
WB,IF
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
Q9H9S0
规格:
目录价
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Description |
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NANOG: Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line. |
References |
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1. Genes Cells. 2006 Sep;11(9):1115-23. 2. Mol Biol Cell. 2007 May;18(5):1543-53. |
Specification |
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Aliases | NANOG |
Entrez GeneID | 79923 |
Swissprot | Q9H9S0 |
clone | 1E6C4 |
WB Predicted band size | 35kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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WB | 1/500 - 1/2000 |
IF | 1/200 - 1/1000 |
ELISA | 1/10000 |