Rabbit Polyclonal Antibody to PRMT5
货号:
P04181
别名:
JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5
应用:
WB,IHC
反应种属:
Human, Mouse, Rat
抗体类型:
Primary antibody
Swissprot:
O14744
规格:
目录价
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Description |
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Protein arginine methyltransferase 5 has been shown to interact with WD repeat-containing protein 77, CLNS1A, Janus kinase 2, SNRPD3 and SUPT5H.Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. |
Specification |
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Aliases | JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5 |
Swissprot | O14744 |
WB Predicted band size | 71 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | Synthetic peptide of human PRMT5 |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
Application |
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WB | 1/200-1/1000 |
IHC | 1/15-1/50 |
ELISA | 1/1000-1/2000 |
Product Image
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Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-3: K562 cells, hela cells, 293T cells, Primary antibody: P04181(PRMT5 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
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The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P04181(PRMT5 Antibody) at dilution 1/20. (Original magnification: ×200)
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The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P04181(PRMT5 Antibody) at dilution 1/20. (Original magnification: ×200)