Rabbit Polyclonal Antibody to RAF1
货号:
P07223
别名:
NS5; CRAF; Raf-1; c-Raf; CMD1NN
应用:
WB,IHC
反应种属:
Human, Mouse, Rat
抗体类型:
Primary antibody
Swissprot:
P04049
规格:
目录价
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Description |
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This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. |
Specification |
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Aliases | NS5; CRAF; Raf-1; c-Raf; CMD1NN |
Swissprot | P04049 |
WB Predicted band size | 73 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | Fusion protein of human RAF1 |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
Application |
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WB | 1/500-1/2000 |
IHC | 1/25-1/100 |
ELISA | 1/5000-1/10000 |
Product Image
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Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Mouse muscle tissue and Hepg2 cell, Primary antibody: P07223(RAF1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
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The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using P07223(RAF1 Antibody) at dilution 1/40. (Original magnification: ×200)