Mouse Monoclonal Antibody to SMAD4
货号：
30091
别名：
JIP; DPC4; MADH4; SMAD4
应用：
WB,IHC,IF,FCM
反应种属：
Human,Mouse
抗体类型：
Primary antibody
Swissprot：
Q13485
规格：
目录价
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Description |
|---|
Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily; SMAD4 is the common SMAD (co-SMAD). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. May act as a tumor suppressor Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. |
References |
|---|
| 1. Am J Hum Genet. 2009 Nov;85(5):628-42. 2. Mol Endocrinol. 2010 Mar;24(3):540-51. |
Specification |
|
|---|---|
| Aliases | JIP; DPC4; MADH4; SMAD4 |
| Entrez GeneID | 4089 |
| Swissprot | Q13485 |
| clone | 4G1C6 |
| WB Predicted band size | 65kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human SMAD4 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Western blot analysis using SMAD4 mouse mAb against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.
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Immunofluorescence analysis of NIH/3T3 cells using SMAD4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Flow cytometric analysis of K562 cells using SMAD4 mouse mAb (green) and negative control (purple).
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using SMAD4 mouse mAb with DAB staining.
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