Mouse Monoclonal Antibody to CCNB1
货号:
30124
别名:
CCNB; CCNB1
应用:
WB,IF,FCM
反应种属:
Human,Rat
抗体类型:
Primary antibody
Swissprot:
P14635
规格:
目录价
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Description |
|---|
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. (provided by RefSeq) It has higher expression in tumor tissues . |
References |
|---|
| 1. Br J Cancer. 2009 Oct 20;101(8):1461-8. 2. Cancer Res. 2010 Feb 1;70(3):1265-74. 3. J Biol Chem. 2010 Jun 4;285(23):17833-45. |
Specification |
|
|---|---|
| Aliases | CCNB; CCNB1 |
| Entrez GeneID | 891 |
| Swissprot | P14635 |
| clone | 5G6 |
| WB Predicted band size | 60kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human CCNB1 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Western blot analysis using CCNB1 mouse mAb against Hela (1) and PC-12 (2) cell lysate.

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Immunofluorescence analysis of Hela cells using CCNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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Flow cytometric analysis of Hela cells using CCNB1 mouse mAb (green) and negative control (purple).




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