Mouse Monoclonal Antibody to CSK
货号：
30117
别名：
MGC117393; CSK
应用：
WB,IF,FCM
反应种属：
Human,Mouse,Monkey,Rat
抗体类型：
Primary antibody
Swissprot：
P41240
规格：
目录价
在线咨询
Description |
|---|
Carboxy-terminal Src kinase (Csk) is a ubiquitously expressed nonreceptor tyrosine kinase that negatively regulates the Src family kinases (SFK) by phosphorylation of the SFK carboxy-terminal tyrosine. Phosphorylated carboxy-terminal tyrosine binds to the SH2 domain of SFK intramolecularly and leads to folding and inactivation of the SFK . This Csk-catalyzed SFK tyrosine phosphorylation is highly specific and exclusive. The SFK carboxy-terminal tyrosine is the only known physiological substrate of Csk .Tissue specificity: Expressed in lung and macrophages. |
References |
|---|
| 1. Nat Genet. 2009 Jun;41(6):677-87. 2. Leuk Res. 2009 Sep;33(9):e168-9. 3. J Hypertens. 2011 Jan;29(1):62-9. |
Specification |
|
|---|---|
| Aliases | MGC117393; CSK |
| Entrez GeneID | 1445 |
| Swissprot | P41240 |
| clone | 5F3 |
| WB Predicted band size | 50kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Monkey,Rat |
| Immunogen | Purified recombinant fragment of human CSK expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
-
Western blot analysis using CSK mouse mAb against NIH/3T3 (1)Hela (2)COS7 (3), Jurkat (4), Raw246.7 (5), A549 (6), HL-60 (7) and PC-12 (8) cell lysate.
-
Immunofluorescence analysis of U251 cells using CSK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
-
Flow cytometric analysis of HL-60 cells using CSK mouse mAb (green) and negative control (purple).
鄂公网安备42018502007531号
