Rabbit Polyclonal Antibody to RNF144B
货号:
P08424
别名:
PIR2; IBRDC2; p53RFP; bA528A10.3
应用:
WB,IHC
反应种属:
Human, Mouse
抗体类型:
Primary antibody
Swissprot:
Q7Z419
规格:
目录价
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Description |
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p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. Another p53 target protein is the p53-inducible RING finger protein (p53RFP), an auto-ubiquitinylated protein acting as an E3 ubiquitin ligase. p53RFP, also designated IBRDC2 in mouse and rat, receives ubiquitin from specific E2 ubiquitin-conjugating enzymes and transfers it to substrates that promote their degradation by the proteasome. p53RFP may mediate re-entry into the cell cycle. |
Specification |
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Aliases | PIR2; IBRDC2; p53RFP; bA528A10.3 |
Swissprot | Q7Z419 |
WB Predicted band size | 34 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse |
Immunogen | Fusion protein of human RNF144B |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
Application |
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WB | 1/200-1/1000 |
IHC | 1/50-1/200 |
ELISA | 1/2000-1/5000 |
Product Image
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Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: TM4 cells, Primary antibody: P08424(RNF144B Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
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The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P08424(RNF144B Antibody) at dilution 1/50. (Original magnification: ×200)
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The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P08424(RNF144B Antibody) at dilution 1/50. (Original magnification: ×200)