Mouse Monoclonal Antibody to PINCH
货号:
30241
别名:
PINCH; PINCH1; PINCH-1; LIMS1
应用:
WB,IF,FCM
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
P48059
规格:
目录价
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Description |
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The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. |
References |
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1. J Biol Chem. 2009 Feb 27;284(9):5836-44. 2. Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20677-82. |
Specification |
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Aliases | PINCH; PINCH1; PINCH-1; LIMS1 |
Entrez GeneID | 3987 |
Swissprot | P48059 |
clone | 5G7 |
WB Predicted band size | 37kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human PINCH expressed in E. Coli. |
Formulation | Ascitic fluid containing 0.03% sodium azide. |
Application |
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IF | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
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Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
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Western blot analysis using PINCH mouse mAb against A549 (1), Jurkat (2), and Hela (3) cell lysate.
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Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and negative control (red).