Mouse Monoclonal Antibody to CCT2
货号：
30274
别名：
CCTB; 99D8.1; PRO1633; CCT-beta; MGC142074; MGC142076; TCP-1-beta
应用：
WB,IF,FCM
反应种属：
Human,Mouse,Monkey,Rat
抗体类型：
Primary antibody
Swissprot：
P78371
规格：
目录价
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Description |
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The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene. |
References |
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| 1. J Biol Chem. 2009 May 29;284(22):14939-48. 2. Mol Cell Proteomics. 2009 Jan;8(1):157-71. |
Specification |
|
|---|---|
| Aliases | CCTB; 99D8.1; PRO1633; CCT-beta; MGC142074; MGC142076; TCP-1-beta |
| Entrez GeneID | 10576 |
| Swissprot | P78371 |
| clone | 5B5C4 |
| WB Predicted band size | 54kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Monkey,Rat |
| Immunogen | Purified recombinant fragment of human CCT2 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
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Western blot analysis using CCT2 mouse mAb against Hela (1), MCF-7 (2), Jurkat (3), T47D (4), K562 (5), A431 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
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Immunofluorescence analysis of 3T3-L1 cells using CCT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Flow cytometric analysis of NIH/3T3 cells using CCT2 mouse mAb (blue) and negative control (red).
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