Mouse Monoclonal Antibody to UBE2I
货号：
30281
别名：
P18; UBC9; C358B7.1
应用：
WB,IHC,IF,FCM
反应种属：
Human,Monkey
抗体类型：
Primary antibody
Swissprot：
P63279
规格：
目录价
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Description |
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The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. |
References |
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| 1. Cell Signal. 2009 Dec;21(12):1935-44. 2. Nat Struct Mol Biol. 2009 Sep;16(9):945-52. |
Specification |
|
|---|---|
| Aliases | P18; UBC9; C358B7.1 |
| Entrez GeneID | 7329 |
| Swissprot | P63279 |
| clone | 1B10 |
| WB Predicted band size | 18kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | Purified recombinant fragment of human UBE2I expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
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Western blot analysis using UBE2I mouse mAb against Hela (1), HepG2 (2), and Cos7 (3) cell lysate.
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Immunofluorescence analysis of HepG2 cells using UBE2I mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Flow cytometric analysis of HepG2 cells using UBE2I mouse mAb (green) and negative control (red).
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Immunohistochemical analysis of paraffin-embedded human prostate tissues using UBE2I mouse mAb with DAB staining.
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Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using UBE2I mouse mAb with DAB staining.
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