Mouse Monoclonal Antibody to RAF1
货号:
30325
别名:
NS5; CRAF; Raf-1; c-Raf
应用:
WB,IHC,FCM
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
P04049
规格:
目录价
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Description |
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This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. |
References |
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1. Am J Hum Genet. 2009 Nov;85(5):628-42. 2. Mol Cancer Res. 2009 Oct;7(10):1635-44. |
Specification |
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Aliases | NS5; CRAF; Raf-1; c-Raf |
Entrez GeneID | 5894 |
Swissprot | P04049 |
clone | 4G4 |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human RAF1 expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
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WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
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Western blot analysis using RAF1 mouse mAb against Hela (1), A431 (2), Cos7 (3) and C6 (4) cell lysate.
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Flow cytometric analysis of Hela cells using RAF1 mouse mAb (green) and negative control (red).
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Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using RAF1 mouse mAb with DAB staining.