Rabbit Polyclonal Antibody to ADO
货号：
P10646
别名：
C10orf22
应用：
WB,IHC
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
Q96SZ5
规格：
目录价
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Description |
|---|
Human thiol dioxygenases include cysteine dioxygenase (CDO, MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO, EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. Mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. The demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity. |
Specification |
|
|---|---|
| Aliases | C10orf22 |
| Swissprot | Q96SZ5 |
| WB Predicted band size | 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ADO |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/1000-1/5000 |
| IHC | 1/25-1/100 |
| ELISA | 1/1000-1/10000 |
Product Image
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Gel: 10%SDS-PAGE, Lysate: 30 μg, Lane: Mouse testis tissue, Primary antibody: P10646(ADO Antibody) at dilution 1/1200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 minutes
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The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P10646(ADO Antibody) at dilution 1/30. (Original magnification: ×200)
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