Mouse Monoclonal Antibody to KEAP1
货号：
30431
别名：
INrf2; KLHL19
应用：
WB,IF,FCM
反应种属：
Human,Mouse
抗体类型：
Primary antibody
Swissprot：
Q14145
规格：
目录价
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Description |
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This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. |
References |
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| 1.Cell Signal. 2010 Nov;22(11):1645-54. 2.Mol Cancer Ther. 2010 Feb;9(2):336-46. |
Specification |
|
|---|---|
| Aliases | INrf2; KLHL19 |
| Entrez GeneID | 9817 |
| Swissprot | Q14145 |
| clone | 1F10B6 |
| WB Predicted band size | 69.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human KEAP1 (AA: 380-624) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| IF | 1/50 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
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Western blot analysis using KEAP1 mouse mAb against NIH3T3 (1), and A549 (2) cell lysate.
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Immunofluorescence analysis of HeLa cells using KEAP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Flow cytometric analysis of HepG2 cells using KEAP1 mouse mAb (green) and negative control (purple).
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