Mouse Monoclonal Antibody to RB1
货号：
30584
别名：
RB; pRb; OSRC; pp110; p105-Rb
应用：
WB,IF,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P06400
规格：
目录价
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Description |
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The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. |
References |
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| 1. Cancer Genet. 2011 Jun;204(6):316-22. 2. J Neuropathol Exp Neurol. 2012 Jan;71(1):83-9. |
Specification |
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|---|---|
| Aliases | RB; pRb; OSRC; pp110; p105-Rb |
| Entrez GeneID | 5925 |
| Swissprot | P06400 |
| clone | 2A11D11 |
| WB Predicted band size | 106.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human RB1 (AA: 745-928) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
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|---|---|
| WB | 1/500 - 1/2000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
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Western blot analysis using RB1 mouse mAb against Jurkat (1) and A431 (2) cell lysate.
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Immunofluorescence analysis of NIH/3T3 cells using RB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of Jurkat cells using RB1 mouse mAb (green) and negative control (red).
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